Immunohistochemical Localization of Transforming Growth Factor Alpha in Regenerating Rat Liver.

Immunohistochemical localization of transforming growth factor alpha in regenerating rat liver.

Immunohistochemical localization of TGF-alpha and cell proliferation kinetics during liver regeneration after two-thirds partial hepatectomy (PH) were investigated. Twenty-four to 72 hr after PH, appreciable increase in the number of TGF-alpha-positive hepatocytes was observed in zones 1 and 2. At the peak at 36 hr, almost all positive cells were stained in their nuclei. Considerable increase i...

The effects of transforming growth factor alpha and somatostatin on regenerating hepatocytes in the rat.

Transforming growth factor alpha (TGF alpha) stimulates DNA synthesis in adult rat hepatocytes, and plays a physiological role after partial hepatectomy by an autocrine mechanism. Somatostatin (SS-14) is a potent inhibitor of gastrointestinal function and inhibits proliferation in various cell types. We examined the proliferative effect of TGF alpha and the inhibitory effect of SS-14 on hepatoc...

TNF-a Regulates Transforming Growth Factor-a Expression in Regenerating Murine Liver and Isolated Hepatocytes

Immunohistochemical Localization of Transforming Growth Factor-/?1, -/?2, and -03 Latency-Associated Peptide in Human Cornea

Methods. Frozen sections obtained from eight human autopsy eyes were placed on gelatincoated slides. After blocking of nonspecific binding sites, the slides were incubated with rabbit polyclonal antibody to the latency-associated peptide (LAP) region of human TGF-01, TGF02, and TGF-/33 precursors, followed by the incubation with biotinylated swine anti-rabbit IgG. Subsequently, a streptavidin-l...

Immunohistochemical localization of transforming growth factor beta isoforms in asbestos-related diseases.

Transforming growth factor beta (TGF-beta), a multifunctional cytokine and growth factor, plays a key role in scarring and fibrotic processes because of its ability to induce extracellular matrix proteins and modulate the growth and immune function of many cell types. These effects are important in inflammatory disorders with fibrosis and cancer. The asbestos-related diseases are characterized ...